New technique and more progress!!!

During this semester I had progress in my project and hence I have learned a new technique. This technique is column chromatography and we use it to isolate compounds from the plant Cundeamor extract, to later analyze by spectroscopic methods. In column chromatography, the stationary phase, a solid adsorbent, for which we use silica, is placed in the column and the mobile phase, a liquid, for which we use various solvents adding by polarity, is added to the top and flows down through the column by gravity.

In the scale 1 to 5, I believe I have a progress of 3 which describes a 50% of the objectives described previously. I’ve been working out the mixture of solvents for optimal separation and has I mentioned before I also have been working with isolation and characterization methods. I still need to learn the technique of preparative TLC and apply all of these to our three principal extracts of Cundeamor: the infusion, the leaves crushed in methanol and the crude by Sohxlet.

Lately, I have encountered some difficulties for the progress of my project because of time issues, because of some random days off that were not part of the academic calendar, and there is not much to do in those cases but to make sure of great performence during the working days.